Fig. 7

Mechanism of DLK1 in AT2 during fibrosis. A-D RNA sequence analysis of primary AT2 cells in AAV-NC and AAV-siDLK1-treated BLM mice (n = 3/group). A The top 20 genes in AT2 cells upregulated by AAV-siDLK1 intervention compared with AAV-NC-treated mice. B, C, D GO, BP and pathway analysis of this sequence. E Relative mRNA expression of CLDN6 in the indicated group. F mRNA expression of related transcription factors that regulated those upregulated genes, such as TTF-1, Gata-6, FoxA2 and NF-kB. G Western blot analysis of TTF-1 and CLDN6 in AAV-NC and AAV-siDLK1-treated BLM mice (n = 4/group). H Western blot analysis of DLK1, TTF-1, a-SMA in Healthy donors and IPF patients. I Co-immunostaining of DLK1 (yellow), SPC (pink), TTF-1(green) and DAPI (blue) in lungs of BLM-induced mice, the scale bar 50um. J Western blot analysis of related signals in A549 cells, including TGF-β/Smad 2/3/AKT/NF-kB. The cells were treated with or without re-DLK1 followed by TGF-β stimulation. K Western blot analysis of related signals in AAV-NC or AAV-siDLK1 treated mice