Fig. 6

Re-DLK1 promoted TGF-β induced fibrosis and si-DLK1 rescued it in vitro. A, B Relative mRNA expression of DLK1 in A549 cells or MRC5 cells treated with either control medium or 10ng/ml TGF-β for 48 h. C Relative mRNA expression of TGF-β1 and fibronectin in A549 cells treated with either control medium or 50ng/ml re-DLK1 for 48 h. D Western blot analysis of collagen1 and DLK1 in MRC5 cells treated with either control medium or 50ng/ml re-DLK1 for 48 h. E Flow chart of in vitro intervention. F Relative mRNA expression of fibronectin, a-SMA, TGF-β1 in MRC5 cells treated with si-DLK1 or 50ng/ml re-DLK1 followed by TGF-β stimulation for 48 h. G Western blot analysis of Collagen1 and a-SMA in MRC5 cells in the indicated group. H Representative fluorescence images of collagen 1 (green) and DAPI (blue) in MRC5 cells treated with or without si-DLK1 followed by TGF-β induction for 48 h, the scale bar 50um. I Relative mRNA expression of TNF-a, fibronectin, TGF-β1 and vimentin in primary lung fibroblasts treated with 0,10,25,50,100,200ng/ml re-DLK1 followed by TGF-β intervention for 48 h