Fig. 3

Alveolar epithelial knockdown of DLK1 alleviated BLM-induced fibrosis. A Schematic diagram of AAV-6 mediated epithelial knockdown of DLK1 following BLM-treated mice. B, C pathological analysis by H&E, Masson and Serius Red staining in the AAV-NC or AAV-siDLK1 treated mice, the scale bar 200um. D Representative Micro-CT images of lungs in control, AAV-NC, and AAV-siDLK1 treated BLM mice. E Relative mRNA expression of collagen1, a-SMA, TGF-β, and fibronectin in control, AAV-NC, and AAV-siDLK1 treated BLM mice. F Western blot analysis of vimentin and a-SMA in AAV-NC and AAV-siDLK1 treated BLM mice. G Representative fluorescence images of collagen1 (green) or a-SMA(green) and DAPI (blue) in control, AAV-NC, and AAV-siDLK1 treated BLM mice the scale bar 50um. Data are presented as mean ± SEM