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Fig. 1 | Respiratory Research

Fig. 1

From: Altered maturation and activation state of circulating monocytes is associated with their enhanced recruitment in pulmonary arterial hypertension

Fig. 1

IPAH and Control monocyte clusters overlap. Peripheral blood mononuclear cells (PBMC) were isolated from peripheral blood of healthy donors (Controls) and idiopathic (I) PAH patients as described in the Methods. (A) Schematic outline of the study design. PBMC were isolated from whole blood of 6 IPAH patients and 6 Controls and processed for single cell sequencing using the 10X Genomics pipeline. Schematic constructed using Biorender.com. (B) UMAP of Control and IPAH PBMC. Major cell types - natural killer (NK) cells, CD4+ and CD8+ T cells, monocytes/ conventional dendritic cells (cDC) and B cells– are grouped with a black outline. (C) Dot plot of gene markers for PBMC cell populations (D) Monocyte subpopulation identification using CD14, CD16 (FCGR3A) and HLA-DRA gene expression. (E) Violin plot CD14, FCGR3A (CD16) and HLA-DRA expression in monocyte clusters

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Respiratory Research

ISSN: 1465-993X

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