Fig. 2

Benzodiazepine-induced change in ASM cell stiffness by magnetic twisting cytometry (MTC). Primary human ASM cells treated with (25, 50, or 100 μM) sulazepam (A), lorazepam (B), or isoproterenol (1 μM) (C) and changes in baseline cell stiffness examined by MTC. Data represent mean ± SE values from n = 50–292 replicates. Statistical significance (P < 0.05) for comparisons between 25 and 50 µM (α), 25 and 100 µM (β) and 50 and 100 µM (δ) measured at 300 secs relative to baseline cell stiffness