Skip to main content
Fig. 6 | Respiratory Research

Fig. 6

From: Intrapleural dual blockade of IL-6 and PD-L1 reprograms CAF dynamics and the tumor microenvironment in lung cancer-associated malignant pleural effusion

Fig. 6

IL-6 in the MPE regulates CAFs and their subtype dynamics. A The mRNA expression of genes including Fap, Pdpn, Pdgfra, Lif and Il6 of pleural tumor nodules of MPE mice treated with anti-PD-L1 and -IL-6 antibody, relative to Actb. B Schematic representation of the co-culture system of CAFs and PBMC. Created with BioRender. CAFs were serum-starved for 12 h and then treated with IL-6 and MPE, respectively, and tocilizumab was added to antagonize IL-6 in MPE. C The percentage of iCAFs (CD90+PDGFRα+HLA-DR−) and myCAFs (CD90+PDGFRα−HLA-DR−) of CAFp1, which was co-cultured with PBMC after MPE only and MPE and tocilizumab together, were quantified by flow cytometry. D The percentage of iCAFs (CD90+PDGFRα+HLA-DR−) and myCAFs (CD90+PDGFRα−HLA-DR−) of CAFp2, which was co-cultured with PBMC after MPE only and MPE and tocilizumab together, were quantified by flow cytometry. F IL-6 levels were assayed in the culture medium of CAFs treated with MPE only and MPE and tocilizumab. G The mRNA expression of genes such as ACTA2, FAP, TAGLN, PDGFRA, IL6 and LIF of CAFs after co-culture with PBMC after MPE only and tocilizumab together, relative to ACTB. PBMC, peripheral blood mononuclear cell; CAFp1, CAFs isolated from patient 1; CAFp2, CAFs isolated from patient 2; TCZ, tocilizumab; NC, negative control

Back to article page

Respiratory Research

ISSN: 1465-993X

Contact us