Fig. 2

IL-6 inhibition and PD-L1 blockade alleviates immunosuppression in MPE. A Representative flow cytometry images of CD8⁺ T cells (CD45⁺CD3⁺CD8⁺) and CD4⁺ T cells (CD45⁺CD3⁺CD4⁺) in MPE mice tumor nodules. The percentage of B CD8⁺ T cells and C CD4⁺ T cells in MPE mice tumor nodules were quantified by flow cytometry. The percentage of D IFN-γ⁺ CD8⁺ T cells (CD45⁺CD3⁺CD8⁺IFN-γ⁺) and E Granzyme B⁺ CD8⁺ T cells (CD45⁺CD3⁺CD8⁺Granzyme B⁺) in tumor nodules were evaluated by flow cytometry. F The percentage of CD8⁺ Tem (CD45⁺CD3⁺CD8⁺CD44⁺CD62L⁻), CD8⁺ Tcm (CD45⁺CD3⁺CD8⁺CD44⁺CD62L⁺) and CD8⁺ naïve T cells (CD45⁺CD3⁺CD8⁺CD44⁻CD62L⁺) in MPE mice tumor nodules were quantified by flow cytometry. G The percentage of CD4⁺ Tem (CD45⁺CD3⁺CD4⁺CD44⁺CD62L⁻), CD4⁺ Tcm (CD45⁺CD3⁺CD4⁺CD44⁺CD62L⁺) and CD4⁺ naïve T cells (CD45⁺CD3⁺CD4⁺CD44⁻CD62L.⁺) in MPE mice tumor nodules were quantified by flow cytometry. H Representative images of IHC staining of CD8, CD4, FOXP3, and F4/80 in MPE mice tumor nodules. Scale bar = 100 µm. All experiments were performed with ≥ 3 biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: not statistically significant. Tem, effector memory T cells; Tcm, central memory T cells; FOXP3, forkhead box protein 3