Fig. 5

Serpinb10 deficiency suppressed M2 polarization in vitro. A Cd206, Arg1 protein levels in baseline and IL-4 stimulated WT and Serpinb10^−/− BMDMs were measured by western blotting. B, C Densitometry assay was performed using ImageJ, and the protein levels of Cd206 (B), Arg1(C) were indexed to Gapdh. D Representative images for co-immunostaining of Serpinb10 and Cd206 in baseline and IL-4 stimulated WT and Serpinb10^−/− BMDM. The nuclei were stained by DAPI. scale bar: 50 μm. E–H Serpinb10 (E), Arg1 (F), Ym1 (G) and Ccl22 (H) mRNA expression in baseline and IL-4 stimulated WT and Serpinb10.^−/− BMDMs were determined by quantitative PCR. Data are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001