Fig. 2

Serpinb10 deficiency attenuates eosinophilic airway inflammation and mucous cell metaplasia in OVA-sensitized and challenged mice. A Schematic overview of mouse experiments. B Representative images of H&E staining of lung sections from WT or Serpinb10^−/− mice sensitized and challenged with saline or OVA. Scale bar: 50 μm. C Representative images of PAS staining of lung sections from WT or Serpinb10^−/− mice sensitized and challenged with saline or OVA. Scale bar: 50 μm. D Lung inflammatory scores were calculated as described in Methods. E PAS scores were calculated as described in Methods. F Pulmonary resistance in response to gradient concentrations of intravenous methacholine in WT mice and Serpinb10^−/− mice sensitized and challenged with saline or OVA. G BAL cell differentiation and counting for macrophages, eosinophils, lymphocytes and neutrophils. n = 6 mice per group. Data are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001