Fig. 5

GGPPS knockout impaired efferocytosis in vitro. Schematic diagrams showing the procedure of determining macrophage efferocytosis in vitro (A). Efferocytosis was determined by flow cytometry quantifying the percentage of CFSE⁺ cells among F4/80⁺ cells 2 h after co-incubation of apoptotic neutrophils and BMDMs (B). The percentage of CFSE⁺ cells among F4/80⁺ BMDMs after co-incubating WT, CKO and CKO + GGOH group (C). Microscopy images of CMTPX-labeled BMDMs and CFSE-labeled apoptotic neutrophils 2 h after co-incubation (D) and the quantification of efferocytotic macrophages (CMTPX⁺CFSE⁺% cells, E). Scale bar = 100 μm. n = 3–6 for each group. Student’s t-test test, **, p < 0.01