Fig. 3

GGPPS knockout impaired efferocytosis in vivo. Efferocytosis was evaluated by flow cytometry quantification of Ly6G⁺ cells in CD11b^hiCD11c^lo and CD11c^hiCD11b^lo macrophages in BALF on Day 3 and 5 (A-C). n = 3–6 for each group. Student’s t-test test, *p < 0.05, **p < 0.01. D Schematic diagrams showing the procedure of determining efferocytosis after i.t. instillation of apoptotic neutrophils. Efferocytosis was determined by flow cytometry quantification of uptake of CFSE-labeled apoptotic cells by macrophages (the percentage of CFSE⁺ cells among F4/80.⁺ cells) 3 h after i.t. instillation of apoptotic human neutrophils into WT or CKO mice (E, F). n = 3–6 for each group. Two-way anova analysis or Student’s t-test test, *, p < 0.05, **, p < 0.01