Fig. 2

GGPPS knockout impaired the resolution of lung injury. Representative image (200 ×) of H&E staining of lung tissue (A) and lung injury score (B) of WT and CKO mice 0d, 3d, and 5d after LPS instillation. Total cell count in BALF was enumerated on an automated cell counter (C). The count of neutrophils (D) and apoptotic neutrophils (E) in BALF of WT and CKO mice on Day 3 and 5. Gating strategy of BALF cells for macrophages (F4/80⁺%, F), recruited macrophages (CD11b^hiCD11c^lo%, F), and resident macrophages (CD11c^hiCD11b^lo%, F). The count of immune cells was calculated based on the total cell count and percentages (D, E, G, H, I). Student’s t-test test, NS, not significant. *, p < 0.05, **, p < 0.01. n = 3–6 for each group