Fig. 1

GGPPS expression level in lung macrophages altered during the development and resolution of lung injury. Representative image (200 ×) of H&E staining of lung tissue (A), lung injury score (B), BALF total cell count (C), neutrophil count (D), macrophage count (E), lymphocyte count (F) and total protein level (G) of C57BL/6 mice after LPS instillation. Immunofluorescence staining for GGPPS (green) in macrophages (F4/80, red) was performed in lung sections on day 0, day 3, and day 5 following LPS instillation (H, I). Yellow spots indicate colocalization of GGPPS in lung macrophages. Representative images were shown. Scale bar = 100 μm. n = 3–6 mice for each group. The mRNA levels of GGPPS in circulating monocytes of mice on day 0, day 3, and day 5 after LPS instillation (J). For Fig J, each dot represents three mice to get enough RNA in monocytes. One-way ANOVA with the Turkey’s multiple comparison test. *, p < 0.05, **, p < 0.01