Fig. 5

(A) Volcano plot depicting the differential expression of lipids between those showing an absolute decline in %FVC < 2.5% (6 M responders) (n = 14) and a decline in %FVC > 2.5% (6 M non-responders) (n = 6) in PF patients at 6 M. (B) Volcano plot depicting the differential expression of lipids between those showing an absolute decline in %FVC < 5% (12 M responders) (n = 15) and a decline in %FVC > 5% (12 M non-responders) (n = 5). Lipids that were up-regulated with less decline in the %FVC are indicated in red, and those that were down-regulated with less decline in the %FVC are indicated in purple. (C) Lipids detected at both 6 M and 12 M. Red lipids were up-regulated and blue lipids were down-regulated in the 6 M responders and 12 M responders. (D) Distribution of lipids extracted at both 6 M and 12 M using box-and-whisker plots. (E) Lipids associated with 24 M survival after starting nintedanib. Lipid samples taken at 6 M were used for analysis. Patients were divided into two groups based on the median level of lipid from all samples at 6 M. The red line indicates patients with a lipid level above the median, and the blue line indicates patients with a lipid level below the median