Fig. 4

Bronchial epithelial cells ETS2 deficiency attenuates OVA-induced oxidative stress injury and mitochondrial dysfunction. (A) Protocol for OVA-sensitised and OVA-challenged chronic mouse model. (B) In OVA-induced asthma, ETS2 mRNA (quantitative reverse transcription polymerase chain reaction) was already upregulated at 14days and peaked at 21 to 28 days. (C) Epithelial cell protein assessed by Western blot at 30 day following OVA-treatment. *P < 0.05 versus saline. (D) Western blot analysis of epithelial neulear and cytoplasmic ETS2 and phosphorylated (p-) ETS2 protein at 30 days following OVA-treatment or saline.*P < 0.05,**P < 0.01 versus saline. Mean ± SD of 3 to 6 animal per group. (F) Representative H&E images for the lung section of the ETS2^fl/fl and ETS2^{fl/fl; Scgb1a1−IRES−cre}(scale bar = 50 μm). (G) Cell counts in BALF from mice. (H) Analyses of the transcription levels for IL-33, IL-25, and TLSP genes in each group through RT-qPCR. mRNA levels were normalised to the housekeeping gene,18s rRNA. (I) ATP content, (J) Relative mtDNA copy number, (K) Western blot image and (L) statistical analysis on relative protein content of PGC-1ɑ,TFAM, MFN2,MFN1 in epithelial cells