Fig. 1

Single-cell transcriptom proliling of airway mucosal cellular in human. (A) Cell populations identified by scRNA-seq in human lung tissues. The uniform manifold approximation and projection (UMAP) of 52,152 single cells from healthy lung (n = 8) and asthma lung tissue (n = 8) samples, and seven major cell clusters identified are labeled. Each dot corresponds to a single cell and is colored according to its cell type. (B) Canonical markers of different cell types were used to identify cell clusters as represented in the UMAP plot (A). (C)Average proportion of the different cell types in healthy lung samples (n = 8) and asthma lung samples (n = 8). (D) Clustering of 52,152 cells from early-stage asthma airway tissues (n = 8) and healthy airway tissues (n = 8). Each dot corresponds to a single cell and is colored according to the type of cell. Of the cells analyzed, 25,397 cells (48.7%) originated from lung cancer tissues, and 26,755 cells (51.3%) originated from healthy lung tissues. (E) Heatmap of cluster marker genes related to (A), with cell lineage of exemplar genes labelled (right). Columns denote cell subtypes and rows denote genes. (F) Sample distribution in each cell subtype. Each bar corresponds to one cell subtype, colored according to samples. (G) Annotation in UMAP plots in healthy airway tissues versus asthma airway tissues. (H) Shown is the heatmap of cluster marker genes of the 7 major cell lineages, with cell lineage of exemplar genes labeled