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Fig. 2 | Respiratory Research

Fig. 2

From: The transcription factor PITX1 cooperates with super-enhancers to regulate the expression of DUSP4 and inhibit pyroptosis in pulmonary artery smooth muscle cells

Fig. 2

Impact of PITX1 on pyroptosis in hypoxia-induced PASMCs. A GSEA: The functions of PITX1 were enriched mainly in the inflammasome signaling pathway under hypoxic conditions. B, C Western blot: The interference efficiency of siPITX1 and the overexpression efficiency of the PITX1 plasmid. D, I Western blot: Representative images and statistical data of CASP1, GSDMD-N, IL-1β, and IL-18 levels. E, J YPI/PI staining: YPI is a green fluorescent dye permeable to the membrane of apoptotic cells, and PI staining of necrotic cells with compromised membrane integrity may result in the emission of red fluorescence. Scale bar: 100 μm. F, K DiO staining: Green fluorescence staining of the cell membrane; and the membranes of live cells exhibit green fluorescence. Scale bar: 50 μm. G, H LDH assay: Determination of the number of dead cells by measuring the amount of LDH released from the membrane of damaged cells. Nor normoxic, Hyp hypoxic, NC noncoding nucleotides, siPITX PITX1 siRNA, and p-PITX PITX1 plasmid. All values are presented as means ± SEMs (*p < 0.05, and **p < 0.01; n ≥ 3)

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Respiratory Research

ISSN: 1465-993X

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