Fig. 2

Comprehensive characterization of plasma-derived EpCAM-specific Exosomes (Epexo). (a) Depicts representative transmission electron microscopy images showcasing the morphological attributes of exosomes with EpCAM expression (Epexo) contrasted against those without (Ep⁻ Exo). (b) Illustrates the size distribution profile for exosomes, as determined through nanoparticle tracking analysis, highlighting uniform particulate dimensions. (c) Presents representative western blot analyses, depicting the protein expression levels of EpCAM and CD63 across different samples, including a positive control-cell lysis, total exosome fractions, and sub-populations of Epexo and Ep⁻ Exo, isolated from four individuals diagnosed with NSCLC. (d) Quantifies and compares the expression levels of EpCAM and CD63 protein within Epexo obtained from patients exhibiting malignant PN (MPN) versus those with benign PN (BPN)