Fig. 7

DHC Alleviates Fibrosis in PCLS. A Schematic diagram illustrating the preparation of hPCLS. B Levels of fibrotic markers in hPCLS treated with a fibrosis-inducing cocktail and DHC (30 µM) for 48 h, tested by Western blot. Each group includes 3 replicates. C Immunofluorescence staining of fibrotic markers in PCLS stimulated with the fibrosis-inducing cocktail and DHC for 48 h, taken at a magnification of 200 ×. D Using the lactate dehydrogenase (LDH) cytotoxicity assay to assess the cell viability of hPCLS, wherein a higher release of LDH from the tissue indicates greater cell damage and lower cell viability. Each group includes 3 replicates. E Levels of ER stress indicators in hPCLS treated with a fibrosis-inducing cocktail and DHC (30 µM) for 48 h, tested by Western blot. Each group includes 3 replicates. Data are presented as mean ± SD. *p < 0.05; **p < 0.01