Fig. 4

Dehydrocorydaline mitigates fibroblast activation. A CCK8 assay to determine the impact of DHC on HPFs viability. Each concentration includes 3 replicates. B The expression levels of fibrotic markers in HPFs treated with recombinant TGF-β1 and DHC (10 µM) for 24 h, tested by Western blot. Each group includes 6 replicates. C–E RT-qPCR analysis results of COL1A1, ACTA2, and FN1 expression in HPFs, following a 24-h stimulation with TGF-β1 and DHC (10 µM). Each group includes 6 replicates. Data are presented as mean ± SD. *p < 0.05; ***p < 0.001