Fig. 3

Impact of dehydrocorydaline on pulmonary fibrosis pathology in mice. A Images of mice lung tissue slices stained with H&E, Masson, and Sirius Red, captured at 50 × original magnification. L-DHC: 5 mg/kg DHC. H-DHC: 10 mg/kg DHC. B Ashcroft scores assessing fibrosis severity. Each group comprised 6 mice. C Quantitative detection results of hydroxyproline content in the lung tissues of mice from each group. The results are expressed as the weight of hydroxyproline (in micrograms) per gram of lung tissue (wet weight). Each group comprised 6 mice. D RT-qPCR results for Col1a1 and Fn1 expression levels in different groups of mice lung tissue. Each group comprised 6 mice. E Collagen 1 and Fibronectin in different groups of mice lung tissues are analyzed using Western blot. Left panel: Typical WB images. Right panel: Bar graphs summarizing the levels of Collagen 1 and Fibronectin across different groups. Each group comprised 6 mice. F Immunofluorescence images of α-SMA, Collagen 1, and Fibronectin in mice lung tissue slices. Images were magnified by 200 × . Data are presented as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001