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Fig. 3 | Respiratory Research

Fig. 3

From: MFGE8 regulates the EndoMT of HLMECs through the BMP signaling pathway and fibrosis in acute lung injury

Fig. 3

Knockdown of MFGE8 exacerbated LPS-induced EndoMT in HLMECs. (A) Microscopy was used to observe the morphology of HLMECs. Magnification, x100; scale bar, 50 μm. (B) A Transwell migration assay was used to examine cell invasion. Magnification, × 100; scale bar, 50 μm. (C) A wound-healing assay was used to assess cell migration. Magnification, x100; scale bar, 50 μm. (D) Fluorescence microscopy was used to examine the protein expression of CD31 and α-SMA. Magnification, × 200; scale bar, 20 μm. (E) Protein and (F) mRNA expression levels of CD31 and α-SMA. Data are presented as the mean ± SD of three repeats. *P < 0.05. siRNA, small interfering RNA; HLMEC, Human lung microvascular endothelial cell; MFGE8, Milk Fat Globule Epidermal Growth Factor 8; LPS, lipopolysaccharide

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Respiratory Research

ISSN: 1465-993X

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