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Fig. 2 | Respiratory Research

Fig. 2

From: MFGE8 regulates the EndoMT of HLMECs through the BMP signaling pathway and fibrosis in acute lung injury

Fig. 2

Analysis of the knockdown efficiency of MFGE8. (A) Western blotting was used to examine the expression of MFGE8 in LPS-induced HLMECs. β-Actin was used as the loading control. (B) mRNA expression levels of MFGE8 in LPS-induced HLMECs. (C and D) For knockdown three specific siRNAs and a non-targeting siRNA control were transfected into HLMECs to knock down MFGE8 expression, and the efficiency of knockdown was assessed using western blotting (C) and RT-qPCR (D). Data are presented as the mean ± SD of three repeats. *P < 0.05 vs. control. HLMEC, Human lung microvascular endothelial cell; MFGE8, Milk Fat Globule Epidermal Growth Factor 8; siRNA, small interfering

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Respiratory Research

ISSN: 1465-993X

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