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Fig. 5 | Respiratory Research

Fig. 5

From: Early secretory antigen target of 6-kDa of Mycobacterium tuberculosis inhibits macrophage apoptosis and host defense via TLR2

Fig. 5

ESAT-6 reduced ROS production and decreased the killing function of THP-1(A). A ESAT-6 reduces ROS in M. tb-infected cells. THP-1(A) cells (1 × 106) were co-incubated with M. tb and different concentrations of ESAT-6 for 4 h and then ROS levels were detected using DCFH-DA. Results were analyzed for MFI results using FlowJo. B Quantitative data of ROS in ESAT-6 and/or mycobacteria stimulated macrophages are shown. C Time and temperature normal kill curve of THP-1(A) cells. The 0 °C and 37 °C groups were set up at 10, 20 and 30 min. The 0 °C groups were used to eliminate the M. tb killed during the first 30 min of phagocytosis. D Quantitative data of Time and temperature normal kill curve of THP-1(A) cells are shown. E ESAT-6 significantly inhibited the killing function of THP-1(A) cells. F The histogram represents the percentage of fluorescence at 0 °C minus the percentage of fluorescence at 37 °C. Results are shown as mean ± SEM. *p < 0.05,**p < 0.01 and ***p < 0.001

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Respiratory Research

ISSN: 1465-993X

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