Table 1 List of antibodies used in flow cytometry analysis
| Â | Antibody for flowcytometry |
|---|---|
Inflammatory cell | anti-mouse CD45 (clone 30-F11) CD11b (clone M1/70) CD11c (clone N418) F4/80 (clone BM8) Ly6c (clone AL-21) MHC class II (clone I-A/I-E) CD170 (clone S17007L) Live/dead aqua (L/D) |
Memory T cell | anti-mouse CD45 (clone 30-F11) CD3 (clone 17A2) CD4 (clone RM4.5) CD8a (clone 53–6.7) CD44 (clone IM7) CD62L (Clone MEL-14) Live/dead aqua (L/D) |
Exhausted T cell | anti-mouse CD45 (clone 30-F11) CD3 (clone 17A2) CD4 (clone RM4.5) CD8a (clone 53–6.7) PD-1 (Clone 29F.1A12) TOX (Clone TXRX10) |
Intracellular cytokine | anti-mouse CD45 (clone 30-F11) CD3 (clone 17A2) CD4 (clone RM4.5) CD8a (clone 53–6.7) IL-4 (Clone 11B11) IFN- γ (Clone XMG1.2) |