Fig. 8

Immunological changes and gut integrity in P. aeruginosa-induced lung infection mouse. (A-C) Representative flow cytometry plots and proportions of Tregs identified as FoxP3⁺ within CD3⁺CD4⁺CD45⁺ T cells in the spleen (A), MLNs (B), and colonic lamina propria (C) across experimental groups (n = 3). (D) Representative flow cytometry plots and proportions of Th17 cells identified as IL-17 A⁺ within CD3⁺CD4⁺CD45⁺ cells in the spleen across groups (n = 3). (E) Representative H&E-stained colon sections from Cont, Ec, Pa, and EcPa groups. Scale bars = 50 μm (n = 5). (F) Disease activity index scores indicating colon injury across groups (n = 5). (G) Histological score of colon injury from H&E-stained sections (n = 5). (H) Submucosa thickness (µm) of the colon in each group (n = 5). Statistics by one-way ANOVA with Tukey’s post-hoc test. Values shown are the mean ± SD. * p < 0.05. ** p < 0.01