Fig. 6
From: NLRP3 deficiency abrogates silica-induced neutrophil infiltration, pulmonary damage and fibrosis
NLRP3 deficiency limits the expression of TGFβ and α-SMA in the lung following silica delivery. (A-D) Nlrp3−/− mice and wildtype (WT) littermates were intranasally administered 2 mg of silica. Control mice received PBS alone. On day 28, lung tissues were formalin-fixed and inflated. (A) Representative images at 20x magnification of immunohistochemical analysis of α-SMA in lung tissue sections. Scale bar 100 μm. (B) Quantification of α-SMA expression determined with ImageJ software. Data are presented as the mean percentage positive pixel count per FOV. (C) Representative images at 20x magnification of immunohistochemical analysis of TGFβ (mature/active form) in lung tissue sections. Scale bar 20 μm. (D) Quantification of TGFβ expression determined with ImageJ software. Data are presented as the mean percentage positive pixel count per FOV. (A-D) Data are presented as mean ± SEM, with each data point representing an individual animal. n = 10–13 per group. Data are pooled from 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, One-way ANOVA