Fig. 4
From: NLRP3 deficiency abrogates silica-induced neutrophil infiltration, pulmonary damage and fibrosis
NLRP3 deficiency diminishes silica-induced neutrophil responses in the lung. (A-I) Nlrp3−/− mice and wildtype (WT) littermates were intranasally administered 2 mg of silica. Control mice received PBS alone. Numbers (#) of (A) total live cells, (B) alveolar macrophages (AMs), (C) Ly6Chi inflammatory macrophages (IMs), and (D) Ly6G+ neutrophils in the BAL on day 3, determined by flow cytometry. (E) Representative flow cytometry gating strategy for Siglec-F− and Siglec-F+ neutrophil subpopulations. Numbers (#) of live (F) Siglec-F− and (G) Siglec-F+ neutrophils. (H) Ratio of Siglec-F− to Siglec-F+ neutrophils. (I) Neutrophil elastase (NE) activity in BAL fluid. The detection limit of the assay was 12.5 pg/mL. (A-I) Data are presented as mean ± SEM, with each data point representing an individual animal. n = 6–10 per group. Data are pooled from 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, One-way ANOVA