Fig. 3
From: NLRP3 deficiency abrogates silica-induced neutrophil infiltration, pulmonary damage and fibrosis
NLRP3 deficiency limits silica-induced IL-1β and IL-18 responses in the lung. (A-G) Nlrp3−/− mice and wildtype (WT) littermates were intranasally administered 2 mg of silica. Control mice received PBS alone. Analysis was performed on day 3. Levels of (A) IL-1β and (B) IL-18 in BAL fluid, determined by ELISA. (C) Immunoblot of equivalent volume of concentrated BAL fluid for pro-IL-1β (p31) and cleaved IL-1β (p17). (D) IL-1β p17 expression represented as optical density per mL of BAL fluid. (E) Immunoblot of lung tissue lysates for pro-IL-1β (p31), IL-1β (p17), and tubulin protein. (F) IL-1β (p17) expression in lung tissues relative to tubulin. (G) Levels of IL-6, TNF, and CCL-2 in BAL fluid determined by cytokine bead array. (A-G) Data are presented as mean ± SEM, with each data point representing an individual animal. n = 5–10 per group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, One-way ANOVA. Data are pooled from 3 independent experiments. The detection limit of each assay is noted as a dotted line