Aging shapes infection profiles of influenza A virus and SARS-CoV-2 in human precision-cut lung slices

Respiratory Research

Table 1 List of qPCR primer and probe sequences and concentrations used

Human/Viral gene	Oligo	Sequence (5'−3')	Final conc. (nM)	Reference
18S rRNA	FW	CGCCGCTAGAGGTGAAATTC	500	[39]
	RV	GGCAAATGCTTTCGCTCTG	500
	P	FAM-TGGACCGGCGCAAGACGGA-BHQ	125
B2M	FW	CTCCGTGGCCTTAGCTGTG	1000	[33]
	RV	TTTGGAGTACGCTGGATAGCCT	1000
IFN-β	FW	CGCCGCATTGACCATCTA	300	[38]
	RV	TTAGCCAGGAGGTTCTCAACAATAGTGTCA	900
	P	FAM-TCAGACAAGATTCATCTAGCACTGGCTGGA-BHQ1	100
IFN-λ1/IL-29	FW	GGACGCCTTGGAAGAGTCACT	300	[46]
	RV	AGAAGCCTCAGGTCCCAATTC	900
	P	FAM-AGTTGCAGCTCTCCTGTCTTCCCCG-BHQ1	100
IFN-λ2/3/IL-28	FW	CTGCCACATAGCCCAGTTCA	300	[46]
	RV	AGAAGCGACTCTTCTAAGGCATCTT	900
	P	FAM-TCTCCACAGGAGCTGCAGGCCTTTA-BHQ1	100
IP10/CXCL10	FW	CCATTCTGATTTGCTGCCTTATC	900	[40]
	RV	GCAGGTACAGCGTACAGTTCT	900
	P	FAM-CTGACTCTAAGTGGCATTCAAGGAGTACCTCTCTC-TAMRA	100
IL-6	FW	CCAGGAGCCCAGCTATGAAC	300	[41]
	RV	CCCAGGGAGAAGGCAACTG	900
	P	FAM-CCTTCTCCACAAGCGCCTTCGGT-TAMRA	100
ACE2	FW	CATTGGAGCAAGTGTTGGATCTT	1000	[44]
	RV	GAGCTAATGCATGCCATTCTCA	1000
	P	FAM-CTTGCAGCTACACCAGTTCCCAGGCA-MGBQ500	125
TMPRSS2	FW	Sequence information not given by the manufacturer		Applied biosystems, TaqMan gene expression assay
	RV			Hs01122322_m1
	P
SARS-CoV-2 E Sarbeco	FW	ACAGGTACGTTAATAGTTAATAGCGT	400	Adapted from [42]
	RV	ATATTGCAGCAGTACGCACACA	600
	P1	FAM-ACACTAGCCATCCTTACTGCGCTTCG-BBQ	125
Flu panAmod	FW	AGATGAGYCTTCTAACCGA	500	[43]
	RV	GCAAAGACATCTTCAAGTYTC	250
	P	FAM-TCAGGCCCCCTCAAAGCCGA-BHQ1	250
Flu M swine	RV	GCAAAGACACYTTCCAGTCTC	250	[43]
CDK2NA (p16)	FW	TGAGCTTTGGTTCTGCCATT	1000	[47]
	RV	AGCTGTCGACTTCATGAC AAG	1000

ISSN: 1465-993X