Fig. 3

A–E. Haemotoxylin and eosin (H&E)- and periodic acid Schiff (PAS)-staining of murine lung tissue sections from OVA-challenged mice with and without TH1579 treatment. (A) Representative images of H&E-stained lungs. Scale bar: 100 μm, 30x magnification. (B) Representative images of PAS-stained lungs. Mucus and mucus containing cells (mainly goblet cells) stain positive for PAS (magenta). Scale bar: 50 μm, 40x magnification. (C) Areas of inflammatory cell infiltrates in H&E stained lung sections were quantified using computer-assisted morphometrical analysis (QuPath). The measured inflammatory area values were normalized to the whole area of each tissue section. (D) Mucus production was analyzed by calculating PAS positive cells (%) in the airways using computer-assisted morphometrical analysis (QuPath). The average percentage of PAS positive cells in four airways per tissue section was measured. (E) Quantification of MUC5AC levels in the bronchoalveolar lavage (BAL) fluid using ELISA. Vehicle/OVA (VO, n = 5), TH1579/OVA (TO, n = 6), dexamethasone/OVA (DO, n = 6), TH1579/PBS (TP, n = 4), and vehicle/PBS (VP, n = 3). The results are displayed as mean ±SD. Statistical comparisons were performed using one-way ANOVA with Dunnett’s post hoc test (****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05)