Fig.5

S1SP intervention resulted in increased levels of inflammatory factors in lung tissue. A Western blotting analysis of the expression levels of IL-6, IL-1β and TNF-α in the lung tissue of mice in each group at 72 h after modeling n = 3. B–D GAPDH was used as an internal control. Quantitative analysis of IL-6, IL-1β and TNF-α n = 3. E–G The results of immunofluorescence were basically consistent with those of Western blot (magnification 200 × , scale bar: 100 µm n = 3). H–I Quantitative analysis of IL-6, IL-1β and TNF-α n = 3. All data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01