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Table 5 Mastermix for cDNA synthesis

From: Cold storage of human precision-cut lung slices in TiProtec preserves cellular composition and transcriptional responses and enables on-demand mechanistic studies

Reagent

Article ID

Final concentration

Random Hexamers

N8080127

10 µM

dNTP Mix

R0192

2 mM

5X First– Strand Buffer

18057018

1X

0,1 M DTT

18057018

40 mM

Reverse Transcriptase

28025013

10 U/µl

RNAse Inhibitor

N8080119

4 U/µl

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Respiratory Research

ISSN: 1465-993X

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