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Table 1 Composition of DMEM/F-12 and cold storage preservation TiProtec

From: Cold storage of human precision-cut lung slices in TiProtec preserves cellular composition and transcriptional responses and enables on-demand mechanistic studies

 

DMEM/F-12

TiProtec

Cl−

126

103.1

Na+

133

16

K+

0.4

93

H2PO4− (41)

 

1

SO42−

0.4

 

Mg2+

1

8

Ca2+

2.22

0.05

Glycine

0.25

10

Alanine

0.05

5

α-Ketoglutarate

 

2

Aspartate

0.05

5

N-Acetylhistidine

0.15

30

Tryptophan

0.05

2

Sucrose

 

20

Glucose

17.5

10

Hepes Buffer

15

 

Deferoxamine*

 

0.1

LK 614*

 

0.02

Other compounds

(cf. to Supplementary Table 1)

 

pH

7.0-7.6

7.0

  1. Concentrations are given in mmol/L; for a simplified comparison of both solutions the main compounds are displayed here. More detailed information about DMEM/F-12, including other compounds, can be found in the Supplementary Table 1. *Not present in TiProtec (-)
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Respiratory Research

ISSN: 1465-993X

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