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Fig. 2 | Respiratory Research

Fig. 2

From: Cold storage of human precision-cut lung slices in TiProtec preserves cellular composition and transcriptional responses and enables on-demand mechanistic studies

Fig. 2

Transcriptional changes in hPCLS after cold storage. A) Principal component analysis for freshly cut hPCLS (T0) and after 7 days of cold storage in DMEM/F-12, TiProtec (-), and TiProtec. Shapes show three (DMEM/F-12) or four (T0, TiProtec (-), TiProtec)) different biological replicates and colors indicate the cold storage solution. B) Venn diagram of differentially expressed genes (DEG) after 7 days of cold storage when compared to T0 baseline control (LFC > 0). C) Cell type signature enrichment analysis of main cellular compartments in hPCLS based on DEG displayed in B. D) Heatmap of DEG after 7 days of cold storage in TiProtec (-) or TiProtec when compared to T0 baseline control (LFC > 1). E) Deregulated pathways in hPCLS after 7 days of cold storage in TiProtec or TiProtec (-) based on DEG from D. F) Deregulated pathways in hPCLS after 7 and 14 days of cold storage in TiProtec based on DEG in comparison to T0 control (LFC > 1).

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Respiratory Research

ISSN: 1465-993X

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