Fig. 4

Characteristics of BALF microbiome in Tumor (for positive Onco-signal samples only), Infection and non-tumor-non-infection (Non) groups. A Relative abundance of top50 bacteria, top15 fungi and all viruses in each group. B Alpha diversity of bacteria, fungi and viruses identified in the three groups of Tumor, Infection and Non. C Specifically enriched microorganisms identified while the group of Tumor compared with each of the other two groups, respectively. Data were analyzed using LEfSe with the LDA threshold = 2. D Clinical relevance of the identified specifically enriched microorganisms with certain clinical parameters. pink: positive correlation; purple: negative correlation. The darker the color, the stronger the correlation. “*” represents p < 0.05. E The fitted smoothing curves of parameters with statistical differences, which was carried out with the Quasi-Poisson model that was performed in R. F Alpha diversity of bacteria, fungi and viruses identified in SCC and ADC. G Specifically enriched microorganisms identified SCC and ADC. Data were analyzed using LEfSe with the LDA threshold = 2. H Clinical relevance of the identified specifically enriched microorganisms in SCC and ADC with certain clinical parameters. pink: positive correlation; purple: negative correlation. The darker the color, the stronger the correlation. “*” represents p < 0.05.