Fig. 6

BRD4 inhibition changed the dynamic enhancer epigenome in M2 AMs. a The left Venn diagram showed enriched genes annotated to BRD4 peaks, H3K27ac peaks in M2 AMs, and their overlapped genes with upregulated genes in M2 macrophage; the right Venn diagram showed overlapped genes between genes associated with ARV825 downregulated BRD4 peaks, genes associated with ARV825 downregulated H3K27ac peaks, and ARV825 downregulated genes in M2 AMs. b The boxplot showed significantly differential gene expression fold changes between genes associated with and not with downregulated BRD4 peaks and H3K27ac peaks in M2 AMs with ARV-825. c GSEA plots indicated genes associated with downregulated BRD4 and H3k27ac peaks are significantly downregulated by ARV-825 in M2 macrophage. d GO analysis of the ARV-825 downregulated genes associated with BRD4 and H3k27ac downregulated peaks in M2 AMs. e Gene tracks showed strong downregulated signals of the M2 marker genes Arg1, Ccl17, Ccr5, and Ccl22 after ARV-825 treatment. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001