Fig. 4

Transcriptome characterization of BRD4 inhibition in M2 AMs. a Pairwise correlations between BRD1, BRD2, BRD3, BRD4, BRD7, BRD8, BRD9, and M2 macrophage expression in Guangzhou and Shenzhen clinical cohorts. b Barplot showed expression of Arg1, Ccl17, and Tfrc (n = 5) in the lung of emphysema mice treated with or without DMSO, ARV825 (10 mg/kg), ARV825 (20 mg/kg), and JQ1 (50 mg/kg). c Heatmap of RNA-seq data of M2 macrophage when using 3 different BRD4 inhibition drugs. d Venn diagrams showing overlapped genes between M2-specific genes and genes downregulated using 3 different BRD4 inhibition drugs. e GSEA enrichment analysis of ARV825 regulated genes against M2-specific gene list. f GO analysis of the differentially expressed mRNAs in BRD4 inhibition drugs of ARV825 downregulated genes. (G) Heatmap analysis of gene expression change in myeloid leukocyte differentiation, leukocyte chemotaxis, and extracellular matrix organization of M2 macrophage when using 3 different BRD4 inhibition drugs. h Barplot showed expression of Arg1, Ccl17 (n = 3) in the AMs stimulated with IL-4 in the treatment with or without DMSO, ARV825 (0.2 µmol), JQ1 (1 µmol). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001