Fig. 7

Cy5-PEG-FUD uptake correlates with fibrosis during the fibrogenic phase of bleomycin-induced pulmonary fibrosis in mice. Mice were treated with bleomycin (1 U/kg, IT) or normal saline before subcutaneous administration of Cy5-PEG-FUD in PEG-FUD (0.1875 mg/kg Cy5-PEG-FUD in 12.5 mg/kg mass dose). One day later, mouse lung tissues were imaged ex vivo using the In Vivo Imaging System and total radiant efficiency quantified. Subsequently, the same tissues were subjected to Masson’s trichrome staining and modified Ashcroft scoring to quantify fibrosis. The total radiant efficiency and Ashcroft scores from each lung were plotted and linear regression modeling was performed on log10 transformed, normalized data combined for all time points (A) and for indicated time points (B-G)