Fig. 2
[64Cu]Cu-PEG-FUD targeting fibrotic PCLS peaks during the fibrogenic phase of bleomycin-induced pulmonary fibrosis. Mice were intratracheally treated with bleomycin (2 U/kg, IT) or normal saline control. Mouse lungs were sectioned into precision cut lung slices (PCLS) and incubated with radiolabeled peptides ([64Cu]Cu-PEG-FUD or [64Cu]Cu-PEG-mFUD control). (A) PCLS generated 14 days after treatment with bleomycin or normal saline (NS) were stained against fibronectin (FN) and PEG. Scale bar = 100 μm. (B) Autoradiography was performed and representative images are displayed with signal adjusted based on normal saline (NS) + [64Cu]Cu-PEG-FUD controls at each time point. (C) Radioactivity on PCLS was quantified via gamma counting. Counts per minute (CPM) were divided by the area and normalized based on NS + [64Cu]Cu-PEG-FUD control signal at each time point. n ≥ 2 mice/ NS groups and n ≥ 2 slices/mouse NS groups. n ≥ 3 mice/ bleomycin groups and n ≥ 1 slices/mouse in bleomycin groups. Data are represented as mean ± SD