Fig. 3

Patients with persistent lung abnormalities have low systemic IL-10 with a lower frequency of B cells and are less able to spontaneously produce immunoglobulins. Cytokines (a) IFN-γ, (b) IL-4, and (c) IL-10 levels were evaluated in plasma patients from FRG (black dots, n = 88) and PLAG (red square, n = 21). Mononuclear cells of FRG (black dots) and PLAG (red square) patients were randomly selected (5 per group), and the phenotype and function were evaluated. (d) the frequency of B-cells (CD19+) was evaluated by flow cytometry. Mononuclear cells were cultured, and (e) IgM and (f) IgG levels were evaluated in the supernatant after 24 h of unstimulated culture. Data are represented as median and IQR values; each symbol represents an individual patient. The Mann–Whitney U test performed statistical comparisons, * p < 0.05, **p < 0.01, **** p < 0.0001