Fig. 1

Gene expression of multiple mediators by RAW 264.7 and MLE-15 cells. Cells were exposed during 1 h to cigarette smoke extract 5% (CSE) and during 4 h to A. fumigatus spores at MOI3 (CSE + AFsp). (a) Fold induction of the gene expression of inflammatory mediators in RAW 264.7 cells. (b) Fold induction of the gene expression of inflammatory mediators in MLE-15 cells (c) Fold induction of the gene expression of inflammatory mediators in co-cultured cells. The mean ± SD is presented, n = 3 in monocultures and n = 4 in co-cultures, *: p < 0.05, **: p < 0.01, ***: p < 0.001