Fig. 7

The effect of fixation method on the wall thickness of intrapulmonary arteries (away from the trachea) across varying diameters. The statistical comparisons of wall thickness in intrapulmonary arteries away from trachea across different diameters between immersion fixation and dual fixation in rats without or with MCT treatment (A-C), and between rats without or with MCT treatment by the same fixation method (A-C). The effect of fixation method and MCT treatment on the alteration of wall thickness of intrapulmonary arteries away from trachea across varying diameters (D). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, n = 24–167 pulmonary vessels from each of 6 rats for each group