Fig. 1

Identification of eosinophil subsets upon subacute HDM + DEP exposure. Female C57BL6/J mice were intranasally exposed to saline or HDM + DEP for 3 weeks. A schematic representation of the experiment. B gating strategy for eosinophil subsets starting from CD45 + CD11c- CD11b + population in blood. C percentage homeostatic eosinophils (hEOS) and inflammatory eosinophils (iEOS) of CD45 + cells in blood. D gating strategy for eosinophil subsets starting from CD45 + CD11c- CD11b + population in lung single cell suspensions. E percentage hEOS and iEOS of CD45 + cells in lung single cell suspensions. Full gating strategies are shown in Supplemental Figure S1 and S2. Results are expressed as mean ± SEM. n = 8 mice/group. *p < 0.05 **p < 0.01