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Fig. 3 | Respiratory Research

Fig. 3

From: Ferritinophagy mediated by the AMPK/ULK1 pathway is involved in ferroptosis subsequent to ventilator-induced lung injury

Fig. 3

Knockdown NCOA4 contributed to reducing ferroptosis caused by ferritinophagy in MLE-12 cells with cyclic overstretching. (A) Immunofluorescence images of the co-localization of intracellular and lysosomal Fe2+. Scale bar: 20 μm. (B) Quantification of the fluorescence intensity of FerroOrange co-localized with Lyso-Tracker Green by ImageJ Fiji software (n = 4). (C) MDA levels in MLE12 cells (n = 4). (D) Immunofluorescence images of C11-BODIPY in MLE12 cells. Scale bar: 50 μm. (E) Percent of the fluorescence intensity of BODIPY C11 and oxidized BODIPY C11 measured by ImageJ Fiji software. (F) Representative Western blotting images of SLC7A11, GPX4, NCOA4, FTH1, and GAPDH in MLE12 cells. (G-J) Relative protein expression levels of SLC7A11, GPX4, NCOA4 and FTH1 to GAPDH (n = 5). Data are expressed as mean ± SD. “*” indicates significant difference between groups (*p < 0.05, ** p < 0.01 or *** p < 0.001)

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Respiratory Research

ISSN: 1465-993X

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