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Fig. 3 | Respiratory Research

Fig. 3

From: T follicular helper cell is essential for M2 macrophage polarization and pulmonary vascular remodeling in hypoxia-induced pulmonary hypertension

Fig. 3

Adoptive transfer of TFH cells induced M2 macrophage polarization in HPH. (a) The percentages of F4/80+CD11c+Fizz1+ M2 macrophages in the spleen and bronchoalveolar lavage of mice. (b, c) Percentages of F4/80+CD11c+Fizz1+ M2 macrophages in the spleen and bronchoalveolar lavage were analysed by histogram. (d) The expression of Fizz1 in bronchoalveolar lavage was verified by immunofluorescence staining. (e) Expression of Fizz1 in bronchoalveolar lavage analysed by western blot. (f) The positive expression of Fizz1 was analyzed by histogram. (g) Quantitative analysis of Fizz1. (h-j) Relative mRNA expression levels of iNOS, IL-12β and MMP9 determined by RT-qPCR in the bronchoalveolar lavage. Values were means ± SD, n = 8. Comparisons were made using the one-way ANOVA with Bonferroni’s post hoc tests. Scale bar = 50 μm. *P < 0.05, ** P < 0.01

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Respiratory Research

ISSN: 1465-993X

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