Fig. 8

ECSN6 treatment prevents RV-A1-induced dissociation of E-cadherin. Mice were infected with sham or RV-A1 and treated with placebo or 40% ECSN6 twice day at 10 h interval for up to 60 h starting from 1 h after RV-A1 infection. (A) At 24 h post-infection, the paraffin sections of snout were prepared and immunostained with E-cadherin and imaged under a fluorescence microscope. Arrow in RV-A1-infected placebo-treated panel denotes dissociation of E-cadherin from the intercellular junctions of nasal epithelium. Images are representative of 3 to 4 mice per group. (B) Density of E-cadherin was measured by Image J and expressed as pixels/50 µm². Data represent median with range and statistical significance was determined by ANOVA on ranks with Tukey post-hoc analysis (n = 3 to 4; *p = < 0.05). (C) Total RNA from sinunasal TRIZOL lysates was isolated and subjected RT-qPCR using gene-specific Taqman assays after 24–72 h post-infection. The mRNA expression was normalized to β-actin. Data represent median with range from 3 experiments with a total of 6 mice per group. ANOVA on ranks with Tukey post-hoc analysis showed no difference between groups