Fig. 2
HAdV-7 infected and induced damage to HULEC-5a cells. a Representative optical microscope images of HULEC-5a cells infected with or without HAdV-7 (MOI = 105) for 24, 48, and 72 h (scale bar: 10 μm). b–d Representative TEM images of (b) viral particles within the nucleus (c, d) and mitochondria structure (scale bar: 2 μm & 500 nm) in HULEC-5a cells infected with or without HAdV-7 (MOI = 105) for 72 h. Red arrow indicates viral particles. e–h Relative protein expression levels of indicated endothelial injury biomarkers in HULEC-5a cells infected with or without HAdV-7 (MOI = 105) for 72 h were determined. i CCK8 analysis of HULEC-5a cells infected with HAdV-7 (MOI = 105) for 24, 48, and 72 h were determined. The data presented are from three independent experiments, with values expressed as standard errors of the means (SEM). *P < 0.05, **P < 0.01, ****P < 0.0001. Compared with the indicated group. HPI: hour post-infection