Fig. 2

Modest reprogramming in sncRNA expression in HLTE in viral and bacterial infections. sncRNA populations were determined by sncRNAseq 24 h after infection with IAV, BCG, and P. aeruginosa, or after mock infection (n = 5 per condition). Results of the DE analysis with DEseq2 are found in Table S1. A Detection efficiency (no. of reads) of all sncRNAs vs. sncRNAs mapping to human genome hg38. B Abundance of the major sncRNA subpopulations miRNA, piRNA, snoRNA, and snRNA. C Abundance of sncRNA subtypes detected at a total of ≥ 20 reads in all samples. D Abundance of differentially expressed sncRNA subtypes (pAdj. ≤ 0.1). E PCA showing poor separation of the four groups based on sncRNA. F–H Volcano plots showing DE sncRNAs (pAdj ≤ 0.1, fold change [FC], ≥|2|). F IAV vs. uninfected tissue. G BCG vs. uninfected tissue. H P. aeruginosa vs. uninfected tissue. I, Hallmark GSEA analysis of predicted targets of DE miRNA in P. aeruginosa infection