Fig. 5
From: Loss of interferon regulatory factor-1 prevents lung fibrosis by upregulation of pon1 expression
Ferroptosis was down regulated in Irf1−/− mice relative to WT mice. KEGG analysis of the differently expressed genes reveals the top 16 pathways of the cellular process; B GO analysis of the top different expressed genes; C GSH levels of WT and Irf1−/− mice after PBS or bleomycin treatment (n = 5 for each group); D MDA levels of WT and Irf1−/− mice after PBS or bleomycin treatment(n = 5 for each group); E–F. Lung tissue lysates from WT and Irf1−/− mice after bleomycin treatment were subjected to Western blot analysis for GPX4, and GAPDH was the standard. G The electron microscopy images after bleomycin intervention in WT mice and IRF1 knockout mice. The left panel represents the WT group, where the mitochondria appear shrunken with a reduction or disappearance of mitochondrial cristae. The right panel depicts the IRF1 knockout mice, where there is an observable improvement in mitochondrial morphology. Statistic analysis was performed by T-test, One-way ANOVA, or Mann–Whitney test. “*” indicates p < 0.05; “**” indicates p < 0.01; “***” indicates p < 0.001; and “****” indicates p < 0.0001